Microbiologia

Engenharia Química e Bioquímica
5.5 ECTS; 2º Ano, 2º Semestre, 30,0 T + 30,0 PL

Lecturer
- Cecília de Melo Correia Baptista

Prerequisites
Not applicable.

Objectives
The students must recognize: the microbial diversity and classification; microbial procaryotic and eucaryotic cell organization, morphology and growth patterns; microbial interactions and role in living systems and biotechnological production.

Program
1 - Introduction to Microbiology
Objectives and history. Insertion of microbial taxonomy in the context of living beings. Major groups of microorganisms, role and importance. Application areas.
2 - Characteristics of different groups of microorganisms
Prokaryotic microorganisms - bacteria and archaebacteria. Eukaryotic microorganisms - fungi and protists (algae and protozoa). Virus - constitution, classification and replication.
3 - Nutrition, growth and reproduction of microorganisms
Nutritional requirements and types. Input of nutrients. Multiplication and death of a microbial population. Generation time and growth rate. Quantitative evaluation of growth. Limiting factors and control. Bacterial metabolism.
4 - Applied Microbiology
Characterization of different microbiological systems - water, soil and air. Food Microbiology. Industrial Microbiology. Prerequisites industrial processes. Main categories of products. Industrial uses of bacteria and fungi.
Laboratorial practice
TP1 - Preparation and sterilization of equipment.
TP2 - Preparation and sterilization of culture media.
TP3 - Aseptic technique and seed handling.
TP4 - Microorganisms in the environment.
TP5 - Counts of viable microorganisms in milk samples.
TP6 - Direct counts of total microorganisms in counting chambers.
TP7 - Staining of bacteria.
TP8 - Culture and microscopic observation of fungi.
TP9 - Water microbiological analysis (membrane filtration technique).

Evaluation Methodology
A - Laboratory ongoing assessment (laboratorial essays -50% and laboratorial written test - 50%).
B - Final theoretical test.
Final classification - 0.4*A + 0.6*B

Bibliography
- Ferreira, W. e Sousa, J. e Lima, N. (2010). Microbiologia. Lisboa: Lidel - Edições Técnicas
- Willey, J. e Sherwood, L. e Woolverton, C. (2008). Prescott, Harley and Klein's Microbiology. New York: McGraw-Hill
- Tortora, G. e Funke, B. e Case, C. (2008). Microbiologia. S. Paulo: Artmed Editora

Method of interaction
Lectures and laboratory classes.

Software used in class
Not applicable.