6 ECTS; 1º Ano, 2º Semestre, 30,0 T + 30,0 PL
- Dina Maria Ribeiro Mateus
An overview of the structure, properties and metabolism of the biological molecules and its functions in living cells. The metabolism of these molecules: carbohydrates, proteins and lipids. Nucleic acids and informational nature of genetic processes.
1 - Biomolecules
1.1 Carbohydrates - definition and classification of carbohydrates . Structure and properties of oses and its derivatives. The glycosidic bond. Holosides and glycosides.
1.2 Lipids - definition, classification and components. Glycerides and phospholipids. Terpenes and steroids. Lipids and biological membranes.
1.3 Proteins - amino acids, classification and properties. Protein binding. Structure, purification and characterization of proteins. Nucleic acid and nucleoproteins.
2 - Introduction to Metabolism
2.1 Enzymes - protein structure and classes.
2.2 Prosthetic groups, cofactors, vitamins and coenzymes.
2.3 Thermodynamics of biological systems. Simplified scheme of cellular metabolism.
2.4 Bioenergetics and the ATP cycle.
3 - Metabolism of biomolecules
3.1 Carbohydrate metabolism. Glycolysis. Fermentations. Pyruvate dehydrogenase system. Krebs and glyoxylate cycles. Respiratory chain and oxidative phosphorylation. Photosynthesis.
3.2 Lipid and protein metabolism.
3.3 Metabolisms integration.
Theme I - Chemical and biochemical characterization of a water
TP2. Dissolved Oxygen
TP3. Chemical Oxygen Demand
TP4. Biochemical Oxygen Demand
Theme II - Characterization of biomolecules
TP5. Purification and characterization of triacylglycerols in natural oils
TP6. Structural analysis of a peptide
TP7. Ascorbic acid assay in biological samples
TP8. DNA extraction from fruit or animal tissue
TP9. Coenzymes spectrophotometric assay.
A - Laboratory continuous assessment (laboratory essays -50% and written laboratory test - 50%).
B - Final theoretical test.
Final classification - 0.4*A + 0.6*B
- Nelson, D. e Cox, M. (2008). Lehninger Principles of Biochemistry. New York: W.H. Freeman & Co
- Voet, J. e Voet, D. (2011). Biochemistry. New York: John Wiley & Sons
- Halpern, M. e Freire, A. e Quintas, A. (2008). Bioquímica - Organização Molecular da Vida. Lisboa: Lidel, Edições Técnicas
Lectures of biomolecules'structure, function and metabolic processes. Laboratory classes depicting extraction, purification and characterization of molecules provided by natural products.
Software used in class